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Sample GSM639298 Query DataSets for GSM639298
Status Public on Feb 09, 2012
Title Identification of miR-133a target genes in HNSCC (IMC3)
Sample type RNA
 
Channel 1
Source name miR-133a transfected in HNSCC cell line (IMC3)
Organism Homo sapiens
Characteristics cell type: head and neck squamous cell carcinoma
cell line: IMC3
Treatment protocol microRNA transfection using Lipofectamine RNiMAX (Invitrogen)
Growth protocol normal cell culture medium RPMI1640 or MEM
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Agilent two-color Low Input Quick Amp Labeling Kit labeling protocol
 
Channel 2
Source name control microRNA transfected cell line
Organism Homo sapiens
Characteristics cell type: head and neck squamous cell carcinoma
cell line: IMC3
Treatment protocol microRNA transfection using Lipofectamine RNiMAX (Invitrogen)
Growth protocol normal cell culture medium RPMI1640 or MEM
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Agilent two-color Low Input Quick Amp Labeling Kit labeling protocol
 
 
Hybridization protocol Agilent two-color gene expression hyb/wash protocol
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 10.7.3.1
Description Differentially expressed genes by miR-133a transfection
Cy5(IMC13)vsCy3(IMCco).txt
Data processing LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
 
Submission date Dec 13, 2010
Last update date Feb 10, 2012
Contact name Naohiko Seki
E-mail(s) naoseki@faculty.chiba-u.jp
Phone 81-43-226-2971
Fax 81-43-227-3442
Organization name Chiba University
Department School of Medicine
Lab Functional Genomics
Street address 1-8-1 Inohana,Chuo-ku
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL10332
Series (1)
GSE26032 Identification of target genes of cancer-related microRNAs in human cancer

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -1.257725144e-001
2 0.000000000e+000
3 0.000000000e+000
4 0.000000000e+000
5 0.000000000e+000
6 0.000000000e+000
7 0.000000000e+000
8 0.000000000e+000
9 0.000000000e+000
10 0.000000000e+000
11 0.000000000e+000
12 0.000000000e+000
13 -2.093335084e-001
14 1.629429696e-001
15 1.514982390e-001
16 5.729008589e-002
17 0.000000000e+000
18 2.141413095e-001
19 1.236469924e-001
20 7.647900459e-002

Total number of rows: 44495

Table truncated, full table size 1006 Kbytes.




Supplementary file Size Download File type/resource
GSM639298.txt.gz 15.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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