|
| Status |
Public on Dec 18, 2013 |
| Title |
PBS treated nasal mucosa of wild type mouse (C56BL/6) |
| Sample type |
RNA |
| |
|
| Source name |
nasal mucosa of wild type mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild-type
strain: C56BL/6
treatment: PBS
|
| Treatment protocol |
Flagellin and other inhibitors were administered intranasally. For intranasal (i.n.) instillations, 25 ul of solution were applied drop-wise into each nostril.
|
| Growth protocol |
Mouse nasal mucosa were harvested from 8wk mouse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
nasal septum and turbinate’s mucosa from each side was gently elevated off the cartilage and bone and total RNA was isolated from these tissues using TRIzol
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Fragmented end-labeled cDNA was hybridized to the GeneChip® Mouse Gene 1.0 ST arrays for 16 hours at 45 “C and 60 rpm as described in the Gene Chip Whole Transcript (WT) Sense Target Labeling Assay Manual (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
PBS treated nasal mucosa of wild type mouse (C56BL/6)
|
| Data processing |
Data was normalized using the RMA(Robust Multi-Average)algorithm implemented in Affymetrix expression console version 1.1 software
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Dec 17, 2010 |
| Last update date |
Jun 16, 2014 |
| Contact name |
JUNGHEE JOO |
| E-mail(s) |
bioplan@yuhs.ac
|
| Phone |
82-2-2228-2667
|
| Fax |
82-2-2228-7998
|
| Organization name |
Yonsei University
|
| Department |
College of Medicine
|
| Lab |
Research Center for Natural Human Defense System,
|
| Street address |
134 Shinchon-dong, Seodaemun-gu
|
| City |
Seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26135 |
Gene analysis of flagellin-treated nasal mucosa of Duox2-/- mice |
|
