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Sample GSM6433311 Query DataSets for GSM6433311
Status Public on Dec 23, 2022
Title Mesothelioma_70455
Sample type genomic
 
Channel 1
Source name normal kidney cortex
Organism Rattus norvegicus
Characteristics genotype: Jcl:SD wild-type
Sex: Male
Sex: Male
Treatment protocol Male and female wild-type and BRCA1+/- rats at 5,6,7 weeks of age were injected intraperitoneally with 3,3,4 mg crocidolite or chrysotile. The rats were euthanized when they were dying.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy3
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
 
Channel 2
Source name Mesothelioma_70455
Organism Rattus norvegicus
Characteristics treatment: Untreated
genotype: Jcl:SD wild-type
treatment: Chrysotile, 10mg
Treatment protocol Male and female wild-type and BRCA1+/- rats at 5,6,7 weeks of age were injected intraperitoneally with 3,3,4 mg crocidolite or chrysotile. The rats were euthanized when they were dying.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy5
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
 
 
Hybridization protocol The labeled DNA was hybridized with Agilent SurePrint G3 Mouse CGH 4x180k microarray at 67°C for 24 hours according to the manufacturer's protocol (Version 8.0).
Scan protocol The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
 
Submission date Aug 05, 2022
Last update date Dec 23, 2022
Contact name Shinya Toyokuni
E-mail(s) akatsuka@med.nagoya-u.ac.jp
Organization name Nagoya University
Department Pathology
Street address 65 Tsuruma-Cho, Showa-Ku
City Nagoya
State/province Aichi
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL10451
Series (1)
GSE210598 Genomic profiles of asbestos-induced mesothelioma developed in wild-type and BRCA1+/- SD rats

Data table header descriptions
ID_REF
VALUE Normalized signal log2 ratio [Cy5/Cy3]

Data table
ID_REF VALUE
23 -0.1765311
24 -0.046346854
25 0.12577373
26 -0.41211012
27 0.19618618
28 -0.10617763
29 0.21204485
30 0.20031312
31 -0.29113448
32 0.047306802
33 -0.5656777
34 0.23081203
35 0.2040912
36 0.17034347
37 0.00282648
38 -0.22673538
39 -0.36737922
40 0.18791556
41 -0.05896547
42 -0.39935964

Total number of rows: 174012

Table truncated, full table size 3042 Kbytes.




Supplementary file Size Download File type/resource
GSM6433311_CCGHarray_70455.txt.gz 49.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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