NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM6433313 Query DataSets for GSM6433313
Status Public on Dec 23, 2022
Title Mesothelioma_60203
Sample type genomic
 
Channel 1
Source name normal kidney cortex
Organism Rattus norvegicus
Characteristics genotype: Jcl:SD wild-type
Sex: Female
Sex: Female
Treatment protocol Male and female wild-type and BRCA1+/- rats at 5,6,7 weeks of age were injected intraperitoneally with 3,3,4 mg crocidolite or chrysotile. The rats were euthanized when they were dying.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy3
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
 
Channel 2
Source name Mesothelioma_60203
Organism Rattus norvegicus
Characteristics treatment: Untreated
genotype: Jcl:SD wild-type
treatment: Chrysotile, 10mg
Treatment protocol Male and female wild-type and BRCA1+/- rats at 5,6,7 weeks of age were injected intraperitoneally with 3,3,4 mg crocidolite or chrysotile. The rats were euthanized when they were dying.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy5
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
 
 
Hybridization protocol The labeled DNA was hybridized with Agilent SurePrint G3 Mouse CGH 4x180k microarray at 67°C for 24 hours according to the manufacturer's protocol (Version 8.0).
Scan protocol The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
 
Submission date Aug 05, 2022
Last update date Dec 23, 2022
Contact name Shinya Toyokuni
E-mail(s) akatsuka@med.nagoya-u.ac.jp
Organization name Nagoya University
Department Pathology
Street address 65 Tsuruma-Cho, Showa-Ku
City Nagoya
State/province Aichi
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL10451
Series (1)
GSE210598 Genomic profiles of asbestos-induced mesothelioma developed in wild-type and BRCA1+/- SD rats

Data table header descriptions
ID_REF
VALUE Normalized signal log2 ratio [Cy5/Cy3]

Data table
ID_REF VALUE
23 -0.30835903
24 -0.35900566
25 0.15249884
26 -0.5990651
27 0.072461575
28 -0.020054687
29 0.13652577
30 0.14730863
31 0.037107203
32 0.048481256
33 0.001004649
34 0.012283584
35 -0.33425182
36 0.09169349
37 -0.05048757
38 -0.05491652
39 0.03138504
40 0.12184635
41 0.151032
42 -0.1216735

Total number of rows: 174012

Table truncated, full table size 3059 Kbytes.




Supplementary file Size Download File type/resource
GSM6433313_CCGHarray_60203.txt.gz 49.7 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap