|
| Status |
Public on Jun 01, 2011 |
| Title |
Stringent |
| Sample type |
RNA |
| |
|
| Source name |
RLM-RACE products reverse transcribed with olfactory receptor degenerate primers and amplified by nested PCR
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
age: 6-8 wk (adult)
tissue: olfactory epithelium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each sample, olfactory epithelia were dissected from five 6-8wk adult C57/Bl6 mice. RNA was extracted using Trizol and RLM-RACE performed using Invitrogen GeneRacer according to the manufacturers instructions with the exception that cDNA was reverse transcribed using degenerate primers against olfactory receptor coding sequences.
|
| Label |
biotin
|
| Label protocol |
5-7ug of DNA was fragmented using UDG/APE and biotinylated using terminal transferase using Affymetrix fragmentation and labeleing kit
|
| |
|
| Hybridization protocol |
5-7ug of DNA was hybridizing at the Stanford PAN facility according to standard Affymetrix protocols
|
| Scan protocol |
Arrays were scanned at the Stanford PAN facility according to standard Affymetrix protocols
|
| Description |
Stringent Hybridization
|
| Data processing |
Data was normalized and analyzed with MAT; all coordinates are mm9/Mouse 2007. Files included here use window 50, maxgap 30, minProbes 5 and default p-value though various parameters were used in analysis. File 'GSE26373_olfrnamedproms.txt' contains final TSS calls for 1085 olfactory receptor genes in mm9 coordinates.
|
| |
|
| Submission date |
Dec 30, 2010 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Eleanor Josephine Clowney |
| E-mail(s) |
eleanor.clowney@ucsf.edu
|
| Organization name |
UCSF
|
| Department |
Anatomy
|
| Lab |
Lomvardas
|
| Street address |
1550 4th St
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL11370 |
| Series (1) |
| GSE26373 |
High-throughput mapping of he promoters of the mouse olfactory receptor genes reveals a new type of mammalian promoters |
|
