|
Status |
Public on Jun 30, 2023 |
Title |
HCC472pre |
Sample type |
RNA |
|
|
Source name |
serum
|
Organism |
Homo sapiens |
Characteristics |
disease/diagnosis: hepatocellular carcinoma sample collection time: before operation molecule subtype: miRNA
|
Treatment protocol |
Blood was collected before surgical treatment.
|
Growth protocol |
Serum was collected from the blood.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from serum was extracted commercially available kit (miReasy mini kit (Qiagen)).
|
Label |
Cy5
|
Label protocol |
Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray Industries Inc., Tokyo, Japan).
|
|
|
Hybridization protocol |
Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
Description |
SH8X435_Cell1
|
Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95%
|
|
|
Submission date |
Aug 29, 2022 |
Last update date |
Jun 30, 2023 |
Contact name |
Satoko Takizawa |
E-mail(s) |
satoko.takizawa.f3@mail.toray
|
Phone |
+81-467-32-9351
|
Organization name |
Toray Industries, Inc.
|
Street address |
6-10-1, Tebiro
|
City |
Kanagawa |
State/province |
Kamakura |
ZIP/Postal code |
2480036 |
Country |
Japan |
|
|
Platform ID |
GPL21263 |
Series (1) |
GSE212211 |
Development of blood biomarkers that distinguish pancreatic cancer from biliary tract cancer |
|