Seedlings were further grown for 20 days in a temperature-controlled glasshouse with a day/night temperature regime of 28/20°C and a 16h photoperiod with additional light of 400 µmol PAR m-2s-1. Micro- and macro-nutrient concentrations in the growth medium were adjusted to 0.7mM K2SO4, 1mM MgSO4.7H2O, 1.65 mM CaCl2, 22.5 mM H2PO4 (macronutrients), and 6.6 mM Mn2+, 4 mM Bo3+, 1.5 mM Cu2+, 1.5 mM Zn2+(micronutrients) and additionally 2.0 g L-1 CaCO3 as pH regulator. After one round of compression and filtering, a constant air flow of 400 mL (compressed air) / liter of solution / minute was applied to each bucket through ‘‘spaghetti” tubes system
Extracted molecule
polyA RNA
Extraction protocol
CTAB LiCl gradient; PolyA+ RNA capture
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
cDNA
Instrument model
454 GS
Description
Sequencing Instrument: First gerenration 454-Machine Place of sequencing: 454 Life Sciences, Branford, CT, USA
