NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM659280 Query DataSets for GSM659280
Status Public on Jun 15, 2011
Title Undifferentiated H1 Cells Canonical Assay 5
Sample type RNA
 
Source name H1 hESC
Organism Homo sapiens
Characteristics cell type: Undifferentiated H1 hESC
Growth protocol H1 human embryonic stem cells were cultured and differentiated as previously described (Bar et al., 2008)
Extracted molecule total RNA
Extraction protocol RNA isolation was performed as described in (Bar et al., 2008)
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization.
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26802 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (hESC)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 41.50074656
hsa-miR-146a 10.69466462
hsa-miR-134 18.08706665
hsa-miR-128 15.62273553
hsa-miR-149 9.462882257
hsa-miR-1308 63.6831655
hsa-miR-200b 24.24827488
hsa-miR-92b 147.4845326
hsa-miR-455-5p 32.87440038
hsa-miR-34c-5p 26.71284718
hsa-miR-99b 99.42189359
hsa-let-7e 11.92657028
hsa-miR-199a-5p 6.999947418
hsa-miR-18b 103.1190143
hsa-miR-509-3p 0.098207632
hsa-miR-320a 6.999947418
hsa-miR-10b 11.92657028
hsa-let-7c 19.31927313
hsa-miR-103 651.5274839
hsa-miR-886-5p 8.231278486

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659280_UndiffCan_5.RCC.gz 6.3 Kb (ftp)(http) RCC
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap