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Sample GSM659283 Query DataSets for GSM659283
Status Public on Jun 15, 2011
Title Undifferentiated H1 Cells Variant 1 Assay 3
Sample type RNA
 
Source name H1 hESC
Organism Homo sapiens
Characteristics cell type: Undifferentiated H1 hESC
Growth protocol H1 human embryonic stem cells were cultured and differentiated as previously described (Bar et al., 2008)
Extracted molecule total RNA
Extraction protocol RNA isolation was performed as described in (Bar et al., 2008)
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization.
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26802 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (hESC)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 5.293178365
hsa-miR-146a 2.44332696
hsa-miR-134 2.44332696
hsa-miR-128 2.44332696
hsa-miR-149 0.092567744
hsa-miR-1308 30.07794395
hsa-miR-200b 1.502349141
hsa-miR-92b 20.54316141
hsa-miR-455-5p 0.034418183
hsa-miR-34c-5p 0.092567744
hsa-miR-99b 10.0565581
hsa-let-7e 6.245319296
hsa-miR-199a-5p 4.341655677
hsa-miR-18b 0.034418183
hsa-miR-509-3p 0.598157393
hsa-miR-320a 0.034418183
hsa-miR-10b 1.502349141
hsa-let-7c 11.00968973
hsa-miR-103 14.82275928
hsa-miR-886-5p 5.293178365

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659283_UndiffV1_3.RCC.gz 6.1 Kb (ftp)(http) RCC
Processed data included within Sample table

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