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Sample GSM659285 Query DataSets for GSM659285
Status Public on Jun 15, 2011
Title Undifferentiated H1 Cells Variant 1 Assay 5
Sample type RNA
 
Source name H1 hESC
Organism Homo sapiens
Characteristics cell type: Undifferentiated H1 hESC
Growth protocol H1 human embryonic stem cells were cultured and differentiated as previously described (Bar et al., 2008)
Extracted molecule total RNA
Extraction protocol RNA isolation was performed as described in (Bar et al., 2008)
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization.
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26802 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (hESC)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 15.53297628
hsa-miR-146a 12.22429807
hsa-miR-134 14.43003999
hsa-miR-128 21.04803716
hsa-miR-149 10.01882613
hsa-miR-1308 30.97588935
hsa-miR-200b 15.53297628
hsa-miR-92b 29.87277015
hsa-miR-455-5p 10.01882613
hsa-miR-34c-5p 13.32714365
hsa-miR-99b 25.460344
hsa-let-7e 17.73893889
hsa-miR-199a-5p 19.94498838
hsa-miR-18b 12.22429807
hsa-miR-509-3p 16.63594457
hsa-miR-320a 12.22429807
hsa-miR-10b 13.32714365
hsa-let-7c 17.73893889
hsa-miR-103 25.460344
hsa-miR-886-5p 18.84195468

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659285_UndiffV1_5.RCC.gz 6.0 Kb (ftp)(http) RCC
Processed data included within Sample table

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