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Sample GSM659299 Query DataSets for GSM659299
Status Public on Jun 15, 2011
Title Differentiated H1 Cells V1 Assay 5
Sample type RNA
 
Source name H1 hESC
Organism Homo sapiens
Characteristics cell type: Differentiated H1 hESC
Growth protocol H1 human embryonic stem cells were cultured and differentiated as previously described (Bar et al., 2008)
Extracted molecule total RNA
Extraction protocol RNA isolation was performed as described in (Bar et al., 2008)
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization.
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26802 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (hESC)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 24.27754489
hsa-miR-146a 28.4213254
hsa-miR-134 9.362093854
hsa-miR-128 22.62006291
hsa-miR-149 11.01893597
hsa-miR-1308 18.47647417
hsa-miR-200b 18.47647417
hsa-miR-92b 22.62006291
hsa-miR-455-5p 20.96260435
hsa-miR-34c-5p 13.50456158
hsa-miR-99b 154.399801
hsa-let-7e 30.07886033
hsa-miR-199a-5p 14.33316659
hsa-miR-18b 8.533787451
hsa-miR-509-3p 9.362093854
hsa-miR-320a 10.1904829
hsa-miR-10b 14.33316659
hsa-let-7c 20.96260435
hsa-miR-103 66.54582769
hsa-miR-886-5p 13.50456158

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659299_DiffV1_5.RCC.gz 6.2 Kb (ftp)(http) RCC
Processed data included within Sample table

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