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Sample GSM659303 Query DataSets for GSM659303
Status Public on Jun 15, 2011
Title Differentiated H1 Cells V2 Assay 3
Sample type RNA
 
Source name H1 hESC
Organism Homo sapiens
Characteristics cell type: Differentiated H1 hESC
Growth protocol H1 human embryonic stem cells were cultured and differentiated as previously described (Bar et al., 2008)
Extracted molecule total RNA
Extraction protocol RNA isolation was performed as described in (Bar et al., 2008)
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using vsn normalization.
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26802 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (hESC)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 13.04382964
hsa-miR-146a 23.09896037
hsa-miR-134 13.95783498
hsa-miR-128 15.78593121
hsa-miR-149 7.561047145
hsa-miR-1308 7.561047145
hsa-miR-200b 19.44235328
hsa-miR-92b 22.18479626
hsa-miR-455-5p 5.734610396
hsa-miR-34c-5p 13.04382964
hsa-miR-99b 53.26806139
hsa-let-7e 25.84148987
hsa-miR-199a-5p 5.734610396
hsa-miR-18b 5.734610396
hsa-miR-509-3p 9.388287573
hsa-miR-320a 6.647686988
hsa-miR-10b 11.21593933
hsa-let-7c 24.01313115
hsa-miR-103 4.821977913
hsa-miR-886-5p 12.1298614

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659303_DiffV2_3.RCC.gz 6.1 Kb (ftp)(http) RCC
Processed data included within Sample table

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