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Sample GSM659362 Query DataSets for GSM659362
Status Public on Jun 15, 2011
Title HCT-116 si ZCCHC6 replicate 1 variant 1 assay
Sample type RNA
 
Source name HCT-116 cells
Organism Homo sapiens
Characteristics cell type: HCT-116
transfection: si ZCCHC6
Treatment protocol Treated with 30 nM Dharmacon SMARTpool siRNAs against enzyme of interest or Cyclophilin B as a negative control
Growth protocol HCT-116 cells grown in McCoy's media with 10% FBS
Extracted molecule total RNA
Extraction protocol RNA was isolated using the miRNeasy RNA isolation kit from Qiagen. 400 ng of RNA was used as input for the nCounter sample prep reaction.
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Description Variant 1
Replicate 1
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using the vsn R package
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26803 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (KD)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 22.18942898
hsa-miR-146a 32.51577124
hsa-miR-134 22.18942898
hsa-miR-128 22.18942898
hsa-miR-149 19.23905324
hsa-miR-1308 382.1374728
hsa-miR-200b 66.44526775
hsa-miR-92b 33.99096495
hsa-miR-455-5p 17.76386775
hsa-miR-34c-5p 19.23905324
hsa-miR-99b 42.84213249
hsa-let-7e 70.8708573
hsa-miR-199a-5p 22.18942898
hsa-miR-18b 16.2886844
hsa-miR-509-3p 20.71424044
hsa-miR-320a 19.23905324
hsa-miR-10b 20.71424044
hsa-let-7c 72.34605389
hsa-miR-103 29.56538493
hsa-miR-886-5p 22.18942898

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659362_zcchc6_1_V1.RCC.gz 6.0 Kb (ftp)(http) RCC
Processed data included within Sample table

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