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Sample GSM659366 Query DataSets for GSM659366
Status Public on Jun 15, 2011
Title HCT-116 si ZCCHC6 replicate 2 variant 2 assay
Sample type RNA
 
Source name HCT-116 cells
Organism Homo sapiens
Characteristics cell type: HCT-116
transfection: si ZCCHC6
Treatment protocol Treated with 30 nM Dharmacon SMARTpool siRNAs against enzyme of interest or Cyclophilin B as a negative control
Growth protocol HCT-116 cells grown in McCoy's media with 10% FBS
Extracted molecule total RNA
Extraction protocol RNA was isolated using the miRNeasy RNA isolation kit from Qiagen. 400 ng of RNA was used as input for the nCounter sample prep reaction.
Label N/A
Label protocol N/A
 
Hybridization protocol Hybrdization reactions were performed according to the manufacturer's instructions (NanoString) with 5 µl of the 5-fold diluted sample preparation reaction. All hybridization reactions were incubated at 65°C for a minimum of 18 h.
Scan protocol Hybridized probes were purified and counted on the nCounter Prep Station and Digital Analyzer (NanoString) following the manufacturer’s instructions. For each assay, a high-density scan (600 fields of view) was performed.
Description Variant 2
Replicate 2
Data processing The data were normalized for lane-to-lane variation with a dilution series of six spike-in positive controls using the vsn R package
 
Submission date Jan 21, 2011
Last update date Jun 15, 2011
Contact name Muneesh Tewari
E-mail(s) mtewari@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Human Biology
Lab Tewari
Street address 1100 Fairview Ave, N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL11642
Series (2)
GSE26803 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity (KD)
GSE26970 Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity

Data table header descriptions
ID_REF
VALUE Normalized miRNA counts

Data table
ID_REF VALUE
hsa-miR-140-5p 12.69164834
hsa-miR-146a 14.34026971
hsa-miR-134 10.21873136
hsa-miR-128 24.23206921
hsa-miR-149 2.800536507
hsa-miR-1308 123.1507566
hsa-miR-200b 16.81321094
hsa-miR-92b 14.34026971
hsa-miR-455-5p 5.273045083
hsa-miR-34c-5p 5.273045083
hsa-miR-99b 8.570136866
hsa-let-7e 19.28615941
hsa-miR-199a-5p 3.624636183
hsa-miR-18b 5.273045083
hsa-miR-509-3p 6.097297668
hsa-miR-320a 9.394431839
hsa-miR-10b 6.921567059
hsa-let-7c 67.92112336
hsa-miR-103 8.570136866
hsa-miR-886-5p 11.0430344

Total number of rows: 125

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM659366_zcchc6_2_V2.RCC.gz 6.0 Kb (ftp)(http) RCC
Processed data included within Sample table

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