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| Status |
Public on May 23, 2023 |
| Title |
Thin_saline treated 3 |
| Sample type |
SRA |
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| Source name |
Uterus
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| Organism |
Mus musculus |
| Characteristics |
tissue: Uterus
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| Treatment protocol |
95% EtOH was instilled into one uterus cavity (TS), and after 4 days, Endo-UdECM (TE) or Whole-UdECM (TE) were treated.
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 (UCSC) whole genome using Bowtie2
Read count extraction using bedtools.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include read count values for each Sample
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| Submission date |
Sep 21, 2022 |
| Last update date |
May 23, 2023 |
| Contact name |
단비 980108 |
| E-mail(s) |
dleksql1198@gmail.com
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| Organization name |
CHA university
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| Department |
Department of biological sciences
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| Lab |
biochemistry
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| Street address |
CHA biocomplex, sampyeongro 335, Seongnam-Si, Kyounggido, Republic of Korea
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| City |
Sungnam |
| ZIP/Postal code |
13493 |
| Country |
South Korea |
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| Platform ID |
GPL19057 |
| Series (1) |
| GSE213906 |
Unraveling the underlying regulatory mechanism of the efficacy of uterine-derived decellularized extracellular matrix for endometrial regeneration and fertility enhancement |
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| Relations |
| BioSample |
SAMN30954983 |
| SRA |
SRX17664442 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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