|
Status |
Public on May 23, 2023 |
Title |
Thin_Endo-UdECM treated 2 |
Sample type |
SRA |
|
|
Source name |
Uterus
|
Organism |
Mus musculus |
Characteristics |
tissue: Uterus
|
Treatment protocol |
95% EtOH was instilled into one uterus cavity (TS), and after 4 days, Endo-UdECM (TE) or Whole-UdECM (TE) were treated.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Illumina Casava1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 (UCSC) whole genome using Bowtie2 Read count extraction using bedtools. Assembly: mm10 Supplementary files format and content: tab-delimited text files include read count values for each Sample
|
|
|
Submission date |
Sep 21, 2022 |
Last update date |
May 23, 2023 |
Contact name |
단비 980108 |
E-mail(s) |
dleksql1198@gmail.com
|
Organization name |
CHA university
|
Department |
Department of biological sciences
|
Lab |
biochemistry
|
Street address |
CHA biocomplex, sampyeongro 335, Seongnam-Si, Kyounggido, Republic of Korea
|
City |
Sungnam |
ZIP/Postal code |
13493 |
Country |
South Korea |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE213906 |
Unraveling the underlying regulatory mechanism of the efficacy of uterine-derived decellularized extracellular matrix for endometrial regeneration and fertility enhancement |
|
Relations |
BioSample |
SAMN30954981 |
SRA |
SRX17664444 |