|
| Status |
Public on Jan 29, 2023 |
| Title |
post burn day 3, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
post day 3 burn
|
| Organism |
Mus musculus |
| Characteristics |
tissue: burn-injured skin
time: post day 3 burn
age: 8-12 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspensions were processed through Chromium 10X to generate single-cell cDNA libraries attached to microbeads
Microbeads were counted, and amplified by PCR, and the 3' end of the cDNA prepared for sequencing using a modified Nextera XT protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.1.0 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Oct 03, 2022 |
| Last update date |
Jan 31, 2023 |
| Contact name |
Steve Potter |
| Organization name |
Cincinnati Children's Hospital Research Foundation
|
| Department |
Developmental Biology
|
| Street address |
240 Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE214717 |
Il-1/MyD88-Dependent G-CSF and IL-6 Secretion Mediates Post Burn Anemia |
|
| Relations |
| BioSample |
SAMN31141395 |
| SRA |
SRX17783788 |
