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Sample GSM6617829 Query DataSets for GSM6617829
Status Public on Oct 03, 2023
Title Cell line-Ctrl-rep1
Sample type RNA
 
Source name TOV112D-control-rep-1
Organism Homo sapiens
Characteristics cell line: TOV112D
cell type: epithelial ovarian cancer
treatment: control
Treatment protocol Human epithelial ovarian cancer cell lines were treated with or without HOXD10
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.)
Label Cy5
Label protocol Cy5
 
Hybridization protocol Fluorescent targets with Cy5 dye (GE Healthcare) were hybridized to the Human Whole Genome OneArray® with Phalanx hybridization buffer using Phalanx Hybridization System.
Scan protocol The slides were scanned using a DNA Microarray Scanner (Model G2505C, Agilent Technologies). The Cy5 fluorescent intensities of each spot were analyzed by GenePix 4.1 software (Molecular Devices).
Data processing The signal intensity was loaded into the Rosetta Resolver System® (Rosetta Biosoftware, USA) for data preprocessing and applied to the 75th percentile for centering normalization. The errors of the sample were estimated by using the error-weighted approach simultaneously. Both the fold change and p-value for pair-wise sample comparison were calculated to evaluate differentially expressed genes (DEGs). Criteria of a fold change of ≥ 2 or ≤ - 2 and p-value < 0.05 are strongly recommended for further analysis.
 
Submission date Oct 06, 2022
Last update date Oct 03, 2023
Contact name ChiaYi Hsu
Organization name Kaohsiung Medical University
Department Graduate Institute of Medicine, College of Medicine
Street address No. 100, Shih-Chung 1
City Kaohsiung
State/province State...
ZIP/Postal code 80708
Country Taiwan
 
Platform ID GPL21282
Series (1)
GSE214910 Gene expression profile analysis of the molecular mechanism of HOXD10 regulating epithelial ovarian cancer cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
PH_hs_0000002 116.0023464
PH_hs_0000003 71.67624654
PH_hs_0000004 7976.811753
PH_hs_0000005 1983.35719
PH_hs_0000006 224.4598247
PH_hs_0000007 767.6903248
PH_hs_0000008 3398.96306
PH_hs_0000009 638.4844593
PH_hs_0000010 39515.30334
PH_hs_0000011 678.0950166
PH_hs_0000012 595.101468
PH_hs_0000013 1038.362466
PH_hs_0000014 358.3812327
PH_hs_0000015 118.8316719
PH_hs_0000016 603.5894445
PH_hs_0000017 52.8140764
PH_hs_0000018 1196.804695
PH_hs_0000019 694.1278612
PH_hs_0000021 403.650441
PH_hs_0000022 153.7266867

Total number of rows: 28264

Table truncated, full table size 673 Kbytes.




Supplementary file Size Download File type/resource
GSM6617829_H001-3140904676.gpr.gz 1.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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