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Sample GSM6617830 Query DataSets for GSM6617830
Status Public on Oct 03, 2023
Title Cell line-Ctrl-rep 2
Sample type RNA
 
Source name TOV112D-control-rep-2
Organism Homo sapiens
Characteristics cell line: TOV112D
cell type: epithelial ovarian cancer
treatment: control
Treatment protocol Human epithelial ovarian cancer cell lines were treated with or without HOXD10
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.)
Label Cy5
Label protocol Cy5
 
Hybridization protocol Fluorescent targets with Cy5 dye (GE Healthcare) were hybridized to the Human Whole Genome OneArray® with Phalanx hybridization buffer using Phalanx Hybridization System.
Scan protocol The slides were scanned using a DNA Microarray Scanner (Model G2505C, Agilent Technologies). The Cy5 fluorescent intensities of each spot were analyzed by GenePix 4.1 software (Molecular Devices).
Data processing The signal intensity was loaded into the Rosetta Resolver System® (Rosetta Biosoftware, USA) for data preprocessing and applied to the 75th percentile for centering normalization. The errors of the sample were estimated by using the error-weighted approach simultaneously. Both the fold change and p-value for pair-wise sample comparison were calculated to evaluate differentially expressed genes (DEGs). Criteria of a fold change of ≥ 2 or ≤ - 2 and p-value < 0.05 are strongly recommended for further analysis.
 
Submission date Oct 06, 2022
Last update date Oct 03, 2023
Contact name ChiaYi Hsu
Organization name Kaohsiung Medical University
Department Graduate Institute of Medicine, College of Medicine
Street address No. 100, Shih-Chung 1
City Kaohsiung
State/province State...
ZIP/Postal code 80708
Country Taiwan
 
Platform ID GPL21282
Series (1)
GSE214910 Gene expression profile analysis of the molecular mechanism of HOXD10 regulating epithelial ovarian cancer cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
PH_hs_0000002 129.1829427
PH_hs_0000003 130.437146
PH_hs_0000004 8306.588636
PH_hs_0000005 2026.792577
PH_hs_0000006 165.5548392
PH_hs_0000007 604.5260037
PH_hs_0000008 4173.988673
PH_hs_0000009 688.5576266
PH_hs_0000010 39296.69865
PH_hs_0000011 277.1789353
PH_hs_0000012 188.1304991
PH_hs_0000013 717.4043031
PH_hs_0000014 443.9879778
PH_hs_0000015 66.47277634
PH_hs_0000016 839.0620259
PH_hs_0000017 66.47277634
PH_hs_0000018 1221.594041
PH_hs_0000019 702.3538632
PH_hs_0000021 600.7633937
PH_hs_0000022 213.2145656

Total number of rows: 28264

Table truncated, full table size 674 Kbytes.




Supplementary file Size Download File type/resource
GSM6617830_H002-3140904677.gpr.gz 1.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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