|
Status |
Public on Oct 03, 2023 |
Title |
Cell line-HOXD10-rep1 |
Sample type |
RNA |
|
|
Source name |
TOV112D-HOXD10-rep-1
|
Organism |
Homo sapiens |
Characteristics |
cell line: TOV112D cell type: epithelial ovarian cancer treatment: HOXD10 overexpression
|
Treatment protocol |
Human epithelial ovarian cancer cell lines were treated with or without HOXD10
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.)
|
Label |
Cy5
|
Label protocol |
Cy5
|
|
|
Hybridization protocol |
Fluorescent targets with Cy5 dye (GE Healthcare) were hybridized to the Human Whole Genome OneArray® with Phalanx hybridization buffer using Phalanx Hybridization System.
|
Scan protocol |
The slides were scanned using a DNA Microarray Scanner (Model G2505C, Agilent Technologies). The Cy5 fluorescent intensities of each spot were analyzed by GenePix 4.1 software (Molecular Devices).
|
Data processing |
The signal intensity was loaded into the Rosetta Resolver System® (Rosetta Biosoftware, USA) for data preprocessing and applied to the 75th percentile for centering normalization. The errors of the sample were estimated by using the error-weighted approach simultaneously. Both the fold change and p-value for pair-wise sample comparison were calculated to evaluate differentially expressed genes (DEGs). Criteria of a fold change of ≥ 2 or ≤ - 2 and p-value < 0.05 are strongly recommended for further analysis.
|
|
|
Submission date |
Oct 06, 2022 |
Last update date |
Oct 03, 2023 |
Contact name |
ChiaYi Hsu |
Organization name |
Kaohsiung Medical University
|
Department |
Graduate Institute of Medicine, College of Medicine
|
Street address |
No. 100, Shih-Chung 1
|
City |
Kaohsiung |
State/province |
State... |
ZIP/Postal code |
80708 |
Country |
Taiwan |
|
|
Platform ID |
GPL21282 |
Series (1) |
GSE214910 |
Gene expression profile analysis of the molecular mechanism of HOXD10 regulating epithelial ovarian cancer cells |
|