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Sample GSM6617831 Query DataSets for GSM6617831
Status Public on Oct 03, 2023
Title Cell line-HOXD10-rep1
Sample type RNA
Source name TOV112D-HOXD10-rep-1
Organism Homo sapiens
Characteristics cell line: TOV112D
cell type: epithelial ovarian cancer
treatment: HOXD10 overexpression
Treatment protocol Human epithelial ovarian cancer cell lines were treated with or without HOXD10
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.)
Label Cy5
Label protocol Cy5
Hybridization protocol Fluorescent targets with Cy5 dye (GE Healthcare) were hybridized to the Human Whole Genome OneArray® with Phalanx hybridization buffer using Phalanx Hybridization System.
Scan protocol The slides were scanned using a DNA Microarray Scanner (Model G2505C, Agilent Technologies). The Cy5 fluorescent intensities of each spot were analyzed by GenePix 4.1 software (Molecular Devices).
Data processing The signal intensity was loaded into the Rosetta Resolver System® (Rosetta Biosoftware, USA) for data preprocessing and applied to the 75th percentile for centering normalization. The errors of the sample were estimated by using the error-weighted approach simultaneously. Both the fold change and p-value for pair-wise sample comparison were calculated to evaluate differentially expressed genes (DEGs). Criteria of a fold change of ≥ 2 or ≤ - 2 and p-value < 0.05 are strongly recommended for further analysis.
Submission date Oct 06, 2022
Last update date Oct 03, 2023
Contact name ChiaYi Hsu
Organization name Kaohsiung Medical University
Department Graduate Institute of Medicine, College of Medicine
Street address No. 100, Shih-Chung 1
City Kaohsiung
State/province State...
ZIP/Postal code 80708
Country Taiwan
Platform ID GPL21282
Series (1)
GSE214910 Gene expression profile analysis of the molecular mechanism of HOXD10 regulating epithelial ovarian cancer cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
PH_hs_0000002 123.2365434
PH_hs_0000003 139.3847801
PH_hs_0000004 8414.081238
PH_hs_0000005 1822.201028
PH_hs_0000006 144.4842233
PH_hs_0000007 1148.224622
PH_hs_0000008 4103.351941
PH_hs_0000009 1404.046687
PH_hs_0000010 18862.8403
PH_hs_0000011 189.5293047
PH_hs_0000012 166.5818104
PH_hs_0000013 624.6817889
PH_hs_0000014 424.1036907
PH_hs_0000015 100.2890491
PH_hs_0000016 1304.607546
PH_hs_0000017 50
PH_hs_0000018 2224.207131
PH_hs_0000019 755.5674971
PH_hs_0000021 354.4113006
PH_hs_0000022 92.63988434

Total number of rows: 28264

Table truncated, full table size 666 Kbytes.

Supplementary file Size Download File type/resource
GSM6617831_H003-3140904678.gpr.gz 1.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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