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Sample GSM6617832 Query DataSets for GSM6617832
Status Public on Oct 03, 2023
Title Cell line-HOXD10-rep2
Sample type RNA
 
Source name TOV112D-HOXD10-rep-2
Organism Homo sapiens
Characteristics cell line: TOV112D
cell type: epithelial ovarian cancer
treatment: HOXD10 overexpression
Treatment protocol Human epithelial ovarian cancer cell lines were treated with or without HOXD10
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.)
Label Cy5
Label protocol Cy5
 
Hybridization protocol Fluorescent targets with Cy5 dye (GE Healthcare) were hybridized to the Human Whole Genome OneArray® with Phalanx hybridization buffer using Phalanx Hybridization System.
Scan protocol The slides were scanned using a DNA Microarray Scanner (Model G2505C, Agilent Technologies). The Cy5 fluorescent intensities of each spot were analyzed by GenePix 4.1 software (Molecular Devices).
Data processing The signal intensity was loaded into the Rosetta Resolver System® (Rosetta Biosoftware, USA) for data preprocessing and applied to the 75th percentile for centering normalization. The errors of the sample were estimated by using the error-weighted approach simultaneously. Both the fold change and p-value for pair-wise sample comparison were calculated to evaluate differentially expressed genes (DEGs). Criteria of a fold change of ≥ 2 or ≤ - 2 and p-value < 0.05 are strongly recommended for further analysis.
 
Submission date Oct 06, 2022
Last update date Oct 03, 2023
Contact name ChiaYi Hsu
Organization name Kaohsiung Medical University
Department Graduate Institute of Medicine, College of Medicine
Street address No. 100, Shih-Chung 1
City Kaohsiung
State/province State...
ZIP/Postal code 80708
Country Taiwan
 
Platform ID GPL21282
Series (1)
GSE214910 Gene expression profile analysis of the molecular mechanism of HOXD10 regulating epithelial ovarian cancer cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
PH_hs_0000002 166.5991554
PH_hs_0000003 139.3240725
PH_hs_0000004 7030.336926
PH_hs_0000005 1516.34718
PH_hs_0000006 145.9585521
PH_hs_0000007 978.9543292
PH_hs_0000008 4856.439097
PH_hs_0000009 1301.095174
PH_hs_0000010 15385.35829
PH_hs_0000011 139.3240725
PH_hs_0000012 140.7984013
PH_hs_0000013 1183.886034
PH_hs_0000014 358.2619006
PH_hs_0000015 89.19689295
PH_hs_0000016 1335.004737
PH_hs_0000017 54.55016593
PH_hs_0000018 1912.94163
PH_hs_0000019 721.683952
PH_hs_0000021 416.4978886
PH_hs_0000022 97.3057014

Total number of rows: 28264

Table truncated, full table size 676 Kbytes.




Supplementary file Size Download File type/resource
GSM6617832_H004-3140904680.gpr.gz 1.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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