GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM66714 Query DataSets for GSM66714
Status Public on Jul 30, 2005
Title Human HL-60 cells exposed to 2.45 GHz radiofrequency for 6 hours
Sample type SAGE
Anchor NlaIII
Tag Count 51601
Tag Length 10
Source name HL-60 cells exposed 6 hours
Organism Homo sapiens
Characteristics 2.45 GHz Radiofrequency fields alter gene expression in cultured human cells.
Treatment protocol Human HL-60 cells were exposed to 2.45 GHz radiofrequency for 6 hours
Extracted molecule polyA RNA
Description The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF fields can cause changes in gene expression. We used the pulsed RF fields at a frequency of 2.45 GHz that is commonly used in telecommunication to expose cultured human HL-60 cells. We used the SAGE (serial analysis of gene expression) method to measure the RF effect on gene expression at the genome level. We observed that 221 genes altered their expression after a 2-hour exposure. The number of affected genes increased to 759 after a 6-hour exposure. Functional classification of the affected genes reveals that apoptosis-related genes were among the up-regulated ones and the cell cycle genes among the down-regulated ones. We observed no significant increase in the expression of heat shock genes. These results indicate that the RF fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism.
FEBS LETTERS, 2005 (in press)
Submission date Jul 28, 2005
Last update date Nov 19, 2008
Contact name San Ming Wang
Phone 224-364-7491
Fax 224-364-5003
Organization name ENH Research Institute, Northwestern University
Department Center for Functional Genomics
Street address 1001 University Place
City Evanston
State/province IL
ZIP/Postal code 60062
Country USA
Platform ID GPL4
Series (1)
GSE3025 2.45 GHz Radiofrequency fields alter gene expression in cultured human cells

Data table header descriptions
COUNT Absolute tag count
TPM tags per million

Data table
CCCATCGTCC 1253 24282.48
TGCACGTTTT 1212 23487.92
GAGGGAGTTT 945 18313.60
GGATTTGGCC 909 17615.94
CCACTGCACT 613 11879.61
GCTCCCCTTT 582 11278.85
CGCTGGTTCC 404 7829.31
CCTAGCTGGA 395 7654.89
CCCCAGCCAG 379 7344.82
TTGGTCCTCT 344 6666.54
GTGAAACCCC 337 6530.88
GGCAAGCCCC 330 6395.22
AGCTCTCCCT 308 5968.88
AGGAAAGCTG 305 5910.74
CTCCTCACCT 277 5368.11
CAATAAATGT 248 4806.11
GAAAAATGGT 237 4592.93
TCAGATCTTT 235 4554.18
CTGTTGATTG 231 4476.66
GTGAAACCCT 226 4379.76

Total number of rows: 17814

Table truncated, full table size 332 Kbytes.

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap