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Status |
Public on Jan 31, 2006 |
Title |
CD4T replicate 2 |
Sample type |
RNA |
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Source name |
Splenic CD4 T cells
|
Organism |
Mus musculus |
Characteristics |
Wild type, C57BL/6, spleen CD4 T cells, CD3+CD4+NK1.1-
|
Extracted molecule |
total RNA |
Extraction protocol |
Approximately 100,000 wild-type CD4 splenic T cells were sorted. Total RNA was extracted from the sorted cells using TRIZOL.
|
Label |
biotin
|
Label protocol |
Approximately 2 µg of amplified RNA was processed to produce biotinylated cRNA targets.
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Hybridization protocol |
standard Affymetrix procedures
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Scan protocol |
standard Affymetrix procedures
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Description |
Wild type, C57BL/6, spleen CD4 T cells, CD3+CD4+NK1.1-
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Data processing |
RMA
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Submission date |
Aug 01, 2005 |
Last update date |
Aug 22, 2005 |
Contact name |
CBDM Lab |
E-mail(s) |
cbdm@hms.harvard.edu
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Phone |
617-432-7747
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Organization name |
Harvard Medical School
|
Department |
Microbiology and Immunobiology
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Lab |
CBDM
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Street address |
77 Avenue Louis Pasteur
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
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Platform ID |
GPL81 |
Series (1) |
GSE3039 |
Innate vs. adaptive lymphocyte gene expression |
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Data table header descriptions |
ID_REF |
|
VALUE |
RMA-calculated Signal intensity |
Data table |
ID_REF |
VALUE |
100001_at |
377.6669917 |
100002_at |
28.33650265 |
100003_at |
15.79747508 |
100004_at |
97.76372705 |
100005_at |
73.75145471 |
100006_at |
11.38690058 |
100007_at |
111.7260879 |
100009_r_at |
9.260056966 |
100010_at |
123.0708889 |
100011_at |
735.1991402 |
100012_at |
3015.423608 |
100013_at |
218.2299221 |
100014_at |
51.86274684 |
100015_at |
18.54022677 |
100016_at |
29.18579459 |
100017_at |
26.56767594 |
100018_at |
133.4456089 |
100019_at |
41.93320821 |
100020_at |
94.24884966 |
100021_at |
15.62829329 |
Total number of rows: 12488
Table truncated, full table size 267 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM66902.CEL.gz |
2.7 Mb |
(ftp)(http) |
CEL |
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