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Sample GSM6690348 Query DataSets for GSM6690348
Status Public on Oct 03, 2023
Title Pt4_normal-tissue1
Sample type SRA
 
Source name normal
Organism Homo sapiens
Characteristics type: tissue
Extracted molecule total RNA
Extraction protocol The EV sheet was fabricated from cellulose nanofibers. The EV capture process from moistened organs using the EV sheet consists of the following steps: EV capture by absorption of ascites on organs, EV storage by drying, washing, and EV release. Then, total RNA was extracted using RNA purification kit (Norgen Biotek Corp, Thorold, Canada). To obtain tissue RNA, tissues were shredded using the gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) and total RNAs was extracted by using the miRNeasy Plus Mini Kit (QIAGEN, Hilden, Germany).
Small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA). Then, PCR products were separated by electrophoresis (120 V, 60 min), and DNA fragments corresponding to 140–160 bp (the length of small non-coding RNA plus the adaptors) were recovered.
 
Library strategy miRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina MiSeq
 
Description 10-PT7-N1_S10_L001_R1_001
Pt4_data file(RPM).xlsx
Data processing CLC Genomics Workbench version 9.5.3 program
Sequence reads were trimmed and mapped to the miRbase 21 database allowing up to two mismatches
Reads per million mapped reads (RPM) method was used for normalization.
Assembly: miRbase 21
 
Submission date Oct 27, 2022
Last update date Oct 03, 2023
Contact name Kosuke Yoshida
E-mail(s) yoshida.kousuke@med.nagoya-u.ac.jp
Organization name Nagoya University
Street address 65 Tsuruma-cho
City Nagoya
State/province Aichi
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL15520
Series (1)
GSE216745 Spatial extracellular vesicle microRNA profiles in ovarian cancer
Relations
BioSample SAMN31493511
SRA SRX18050840

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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