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|
| Status |
Public on Jan 31, 2006 |
| Title |
NKT replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Splenic NKT cells
|
| Organism |
Mus musculus |
| Characteristics |
Wild type, C57BL/6, spleen NKT cells, CD3+CD4+NK1.1+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 100,000 wild-type splenic NKT cells were sorted. Total RNA was extracted from the sorted cells using TRIZOL.
|
| Label |
biotin
|
| Label protocol |
Approximately 2 µg of amplified RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Wild type, C57BL/6, spleen NKT cells, CD3+CD4+NK1.1+
|
| Data processing |
RMA
|
| |
|
| Submission date |
Aug 01, 2005 |
| Last update date |
Aug 22, 2005 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE3039 |
Innate vs. adaptive lymphocyte gene expression |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
RMA-calculated Signal intensity |
| Data table |
| ID_REF |
VALUE |
| 100001_at |
464.4950217 |
| 100002_at |
27.22663687 |
| 100003_at |
18.67178326 |
| 100004_at |
93.83319072 |
| 100005_at |
77.3669236 |
| 100006_at |
10.53313087 |
| 100007_at |
107.5432977 |
| 100009_r_at |
10.06009043 |
| 100010_at |
140.4032497 |
| 100011_at |
945.391568 |
| 100012_at |
3293.466051 |
| 100013_at |
243.5396179 |
| 100014_at |
47.43308907 |
| 100015_at |
16.38275419 |
| 100016_at |
27.33832239 |
| 100017_at |
21.65793489 |
| 100018_at |
132.4818865 |
| 100019_at |
42.18250311 |
| 100020_at |
107.2867507 |
| 100021_at |
14.70023748 |
Total number of rows: 12488
Table truncated, full table size 267 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM66906.CEL.gz |
2.7 Mb |
(ftp)(http) |
CEL |
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