|
| Status |
Public on Jan 31, 2006 |
| Title |
CD8abT replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
RTOC CD8abT cells
|
| Organism |
Mus musculus |
| Characteristics |
CD8 alpha beta RTOC cells selected by low-dose agonist (100 nM Smcy). TCRb+CD8a+CD8b+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 50,000 CD8ab T cells were sorted. Total RNA was extracted from the sorted cells using TRIZOL.
|
| Label |
biotin
|
| Label protocol |
Approximately 2 µg of amplified RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
CD8 alpha beta RTOC cells selected by low-dose agonist (100 nM Smcy). TCRb+CD8a+CD8b+
|
| Data processing |
RMA
|
| |
|
| Submission date |
Aug 01, 2005 |
| Last update date |
Aug 22, 2005 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE3039 |
Innate vs. adaptive lymphocyte gene expression |
|
