|
Status |
Public on Aug 13, 2023 |
Title |
interscapular brown adipose tissue |
Sample type |
RNA |
|
|
Source name |
interscapular brown adipose tissue
|
Organism |
Mus musculus |
Characteristics |
genotype: C57BL/6
|
Treatment protocol |
No treatment
|
Growth protocol |
8-week-old male C57BL/6J mices are raised under room temperature
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from each pooled sample was isolated using the TRIzol method for Affymetrix miRNA microarray analysis.
|
Label |
biotin
|
Label protocol |
Procedures were performed as described on the web site of CapitalBio.
|
|
|
Hybridization protocol |
Biotin-labelled miRNAs were used for hybridization on each miRNA microarray chip containing probes in triplicate.
|
Scan protocol |
standard Affymetrix protocol
|
Description |
interscapular brown adipose tissue from C57BL/6 mouse housed at room temperature
|
Data processing |
Raw data were normalized to U6 and analysed using GenePix Pro 4.0 software
|
|
|
Submission date |
Nov 03, 2022 |
Last update date |
Aug 13, 2023 |
Contact name |
Xiaohong Jiang |
E-mail(s) |
xiaohongjiang@outlook.com
|
Organization name |
Nanjing University
|
Street address |
163 Xianlin Rd.
|
City |
Nanjing |
ZIP/Postal code |
210000 |
Country |
China |
|
|
Platform ID |
GPL14613 |
Series (1) |
GSE217222 |
miRNA expression from mouse adipose tissues |
|