|
| Status |
Public on Aug 13, 2023 |
| Title |
subcutaneous white adipose tissue |
| Sample type |
RNA |
| |
|
| Source name |
subcutaneous white adipose tissue
|
| Organism |
Mus |
| Characteristics |
genotype: C57BL/6
|
| Treatment protocol |
No treatment
|
| Growth protocol |
8-week-old male C57BL/6J mices are raised under room temperature
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each pooled sample was isolated using the TRIzol method for Affymetrix miRNA microarray analysis.
|
| Label |
biotin
|
| Label protocol |
Procedures were performed as described on the web site of CapitalBio.
|
| |
|
| Hybridization protocol |
Biotin-labelled miRNAs were used for hybridization on each miRNA microarray chip containing probes in triplicate.
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
subcutaneous white adipose tissue from C57BL/6 mouse housed at room temperature
|
| Data processing |
Raw data were normalized to U6 and analysed using GenePix Pro 4.0 software
|
| |
|
| Submission date |
Nov 03, 2022 |
| Last update date |
Aug 13, 2023 |
| Contact name |
Xiaohong Jiang |
| E-mail(s) |
xiaohongjiang@outlook.com
|
| Organization name |
Nanjing University
|
| Street address |
163 Xianlin Rd.
|
| City |
Nanjing |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE217222 |
miRNA expression from mouse adipose tissues |
|
