|
Status |
Public on Aug 06, 2005 |
Title |
Arabidopsis_Stigma03 |
Sample type |
RNA |
|
|
Source name |
pistil
|
Organism |
Arabidopsis thaliana |
Characteristics |
Landsberg erecta (Ler)
|
Growth protocol |
Ovary tissues were isolated from pistils after emasculating stage 8 buds (Bowman, 1994) of Landsberg erecta (Ler) flowers. After one day of growth, pistils were harvested. Stigma was seperated from ovaries using superfine tip scissors and flash frozen in liquid nitrogen until RNA harvesting.
|
Extracted molecule |
total RNA |
Label |
Bioarray High Yield RNA Transcript Labeling Kit
|
|
|
Hybridization protocol |
Affymetrix GeneChip technology
|
Description |
Ovary tissues were isolated from pistils after emasculating stage 8 buds (Bowman, 1994) of Landsberg erecta (Ler) flowers. After one day of growth, pistils were harvested. Stigma was seperated from ovaries using superfine tip scissors and flash frozen in liquid nitrogen until RNA harvesting.
|
Data processing |
statistical averaging of probes using Affymetrix software
|
|
|
Submission date |
Aug 04, 2005 |
Last update date |
Aug 28, 2018 |
Contact name |
Daphne Preuss |
E-mail(s) |
dpreuss@midway.uchicago.edu, rob.swanson@valpo.edu, tclark@ittc.ku.edu
|
Organization name |
The University of Chicago
|
Department |
Molecular Genetics and Cell Biology
|
Street address |
1103 E. 57th Street
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
66037 |
Country |
USA |
|
|
Platform ID |
GPL198 |
Series (1) |
GSE3056 |
Arabidopsis Pollination Study |
|
Relations |
Reanalyzed by |
GSE119083 |