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Sample GSM6720284 Query DataSets for GSM6720284
Status Public on Dec 22, 2022
Title healthy, liver, ctrl1
Sample type SRA
 
Source name liver
Organism Macaca mulatta
Characteristics tissue: liver
infection: healthy control
Sex: male
animal: healthy animal #1
Extracted molecule polyA RNA
Extraction protocol Tissue blocks that stored at -80 refrigerators were homogenized and performed density gradient centrifugation to cleanup, single-nucleus suspension is the final product for 10X Genomics system.
Library construction was carried out in according to manufacturer's protocol.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description healthy animal #1, male
Data processing Illumina Casava1.8 software used for basecalling
Reads were aligned to macaca mulatta pre-mRNA reference genome by Cell Ranger count (v3.1.0).
Alignment results of macaque data were aggragated by Cell Ranger aggr (v3.1.0).
Aggragated data of macaque were read into scanpy.
Assembly: macaca mulatta (release 95) pre-mRNA
Supplementary files format and content: The file Macaca_global_integration.h5ad is formatted by h5ad (created by scanpy), contains all samples in this study.
 
Submission date Nov 07, 2022
Last update date Dec 22, 2022
Contact name Qiang Ma
E-mail(s) maqiang16@mails.ucas.ac.cn
Organization name Institute of Biophysics, Chinese Academy of Sciences
Street address Datun Road, #15
City Beijing
ZIP/Postal code 100101
Country China
 
Platform ID GPL27943
Series (1)
GSE217483 Single-nucleus transcriptomic profiling of multiple organs from rhesus macaque model with SARS-CoV-2 infection
Relations
BioSample SAMN31647833
SRA SRX18200600

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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