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Sample GSM6720286 Query DataSets for GSM6720286
Status Public on Dec 22, 2022
Title healthy, cerebrum, ctrl1
Sample type SRA
Source name cerebrum
Organism Macaca mulatta
Characteristics tissue: cerebrum
infection: healthy control
Sex: male
animal: healthy animal #1
Extracted molecule polyA RNA
Extraction protocol Tissue blocks that stored at -80 refrigerators were homogenized and performed density gradient centrifugation to cleanup, single-nucleus suspension is the final product for 10X Genomics system.
Library construction was carried out in according to manufacturer's protocol.
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description healthy animal #1, male
Data processing Illumina Casava1.8 software used for basecalling
Reads were aligned to macaca mulatta pre-mRNA reference genome by Cell Ranger count (v3.1.0).
Alignment results of macaque data were aggragated by Cell Ranger aggr (v3.1.0).
Aggragated data of macaque were read into scanpy.
Assembly: macaca mulatta (release 95) pre-mRNA
Supplementary files format and content: The file Macaca_global_integration.h5ad is formatted by h5ad (created by scanpy), contains all samples in this study.
Submission date Nov 07, 2022
Last update date Dec 22, 2022
Contact name Qiang Ma
Organization name Institute of Biophysics, Chinese Academy of Sciences
Street address Datun Road, #15
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL27943
Series (1)
GSE217483 Single-nucleus transcriptomic profiling of multiple organs from rhesus macaque model with SARS-CoV-2 infection
BioSample SAMN31647831
SRA SRX18200602

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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