|
| Status |
Public on Jan 25, 2023 |
| Title |
#115, CEACAM1+ Treg |
| Sample type |
SRA |
| |
|
| Source name |
Tumor infiltrating lymphocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tumor infiltrating lymphocytes
cell line: primary
cell type: regulatory T cell
cell subtype: CEACAM1+ Treg
treatment: none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the sorted cells using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara).
Libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
FASTQ files were mapped to the human genomic DNA reference (GRCh37/hg19) using Hisat2
StringTie was used to quantify gene expression based on the aligned reads.
Supplementary files format and content: hg19
Supplementary files format and content: tab-delimited text files include TPM expression for each Sample
|
| |
|
| Submission date |
Nov 09, 2022 |
| Last update date |
Jan 25, 2023 |
| Contact name |
Seung Hyuck Jeon |
| E-mail(s) |
jeonseunghyuck@gmail.com
|
| Organization name |
Korea Advanced Institute of Science and Technology
|
| Street address |
291 Daehak-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE217613 |
RNA-seq of CEACAM1- and CEACAM1+ regulatory T cells from tumor in patients with renal cell carcinoma |
|
| Relations |
| BioSample |
SAMN31669980 |
| SRA |
SRX18222174 |
