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Sample GSM6729235 Query DataSets for GSM6729235
Status Public on Oct 04, 2023
Title D1
Sample type RNA
 
Source name plasma exosomes of patients with CSVD
Organism Homo sapiens
Characteristics sample type: circulating plasma exosomes
Treatment protocol Exosome was isolated using total exosome isolation (from plasma) reagent (cat no.4484451, Thermo Fisher Scientific, Inc.).Store the isolated exosomes in a refrigerator at -80 °C pending RNA extraction.
Growth protocol All subjects had fasting venous blood drawn within 24h of admission, and the blood specimens were immediately centrifuged and stratified (300 g, 4°C, 10 min). The upper layer of plasma was aspirated, diluted to 10 ml with PBS buffer, then stratified by high-speed centrifugation (2000 g, 4°C, 10 min), and the sediment at the bottom of the tube was discarded. The collected plasma was stored in a refrigerator at -80 ℃ pending extraction of exosomes.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA from exosomes was performed according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array in GeneChip Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Nov 13, 2022
Last update date Oct 04, 2023
Contact name Kejin Gao
E-mail(s) 2020026122@qdu.edu.cn
Organization name The Affiliated Hospital of Qingdao University
Street address NO.59 hai er Rd, Qingdao, China
City QingDao
State/province Shan Dong
ZIP/Postal code 266061
Country China
 
Platform ID GPL21572
Series (1)
GSE217872 A retrospective clinical study to explore diagnostically significant markers in circulating exosomes of patients with cerebral small vessels disease (CSVD)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
20534360 1.44114
20536883 1.25328
20534319 1.34413
20500780 1.10886
20525582 1.5935
20525387 1.78185
20537076 1.28559
20538248 1.24751
20536784 1.24704
20515545 1.33641
20532987 1.2631
20534079 1.25398
20536803 1.36052
20524999 1.16979
20532911 1.38175
20511559 1.10769
20503883 0.874004
20501232 1.14387
20500114 1.58187
20535876 1.12144

Total number of rows: 6658

Table truncated, full table size 110 Kbytes.




Supplementary file Size Download File type/resource
GSM6729235_D1_miRNA-4_0_.CEL.gz 682.3 Kb (ftp)(http) CEL
Processed data included within Sample table

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