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Sample GSM6729238 Query DataSets for GSM6729238
Status Public on Oct 04, 2023
Title D4
Sample type RNA
 
Source name plasma exosomes of patients with CSVD
Organism Homo sapiens
Characteristics sample type: circulating plasma exosomes
Treatment protocol Exosome was isolated using total exosome isolation (from plasma) reagent (cat no.4484451, Thermo Fisher Scientific, Inc.).Store the isolated exosomes in a refrigerator at -80 °C pending RNA extraction.
Growth protocol All subjects had fasting venous blood drawn within 24h of admission, and the blood specimens were immediately centrifuged and stratified (300 g, 4°C, 10 min). The upper layer of plasma was aspirated, diluted to 10 ml with PBS buffer, then stratified by high-speed centrifugation (2000 g, 4°C, 10 min), and the sediment at the bottom of the tube was discarded. The collected plasma was stored in a refrigerator at -80 ℃ pending extraction of exosomes.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA from exosomes was performed according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array in GeneChip Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Nov 13, 2022
Last update date Oct 04, 2023
Contact name Kejin Gao
E-mail(s) 2020026122@qdu.edu.cn
Organization name The Affiliated Hospital of Qingdao University
Street address NO.59 hai er Rd, Qingdao, China
City QingDao
State/province Shan Dong
ZIP/Postal code 266061
Country China
 
Platform ID GPL21572
Series (1)
GSE217872 A retrospective clinical study to explore diagnostically significant markers in circulating exosomes of patients with cerebral small vessels disease (CSVD)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
20534360 1.3261
20536883 1.24846
20534319 1.06472
20500780 0.975463
20525582 1.34148
20525387 1.68126
20537076 1.25598
20538248 1.59651
20536784 1.04841
20515545 1.18162
20532987 1.41922
20534079 1.22835
20536803 1.22835
20524999 1.10771
20532911 1.79991
20511559 1.15212
20503883 1.35917
20501232 1.44441
20500114 1.09775
20535876 1.52893

Total number of rows: 6658

Table truncated, full table size 110 Kbytes.




Supplementary file Size Download File type/resource
GSM6729238_D4_miRNA-4_0_.CEL.gz 677.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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