NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM6734868 Query DataSets for GSM6734868
Status Public on Apr 30, 2023
Title Paternal obesity: Liver F1, biological rep3
Sample type RNA
 
Source name C57BL/6J
Organism Mus musculus
Characteristics tissue: Liver
treatment: Offspring of HFD male
Treatment protocol 14 weeks old male mice offspring from control or obese males and control females (named CT and F1, respectively) were analized. Prior to the crossing, 8 weeks old male mice were fed chow diet or a high-fat diet (45% lipids) for 12 weeks.
Growth protocol 14 weeks old male mice offspring from control or obese males and control females (named CT and F1, respectively) were analized. Prior to the crossing, 8 weeks old male mice were fed chow diet or a high-fat diet (45% lipids) for 12 weeks.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen liver and eWAT with miRNeasy Mini Kit and treated with DNAse, following standard procedures
Label biotin
Label protocol 150 ng of good quality RNA samples (RIN >8) with a Ratio 260/280 = 1.8-2 were used. Fragmentation and Biotin labelling of ss-cDNA was prepared according to Affymetrix WT PLUS Reagent Kit user guide, using an automated system (Biomek FX System, Beckman Coulter)
 
Hybridization protocol Following fragmentation and terminal labeling, ss-cDNA was hybridized for 17hr at 45ºC on Clariom™ S HT, mouse array plate, using the automated GeneTitan System (Affymetrix), which includes the hybridization oven, Fluidic Station and Scanner
Scan protocol Chips were scanned using the Scanner of the GeneTitan System
Description Liver F1, biological rep3
Gene expression data from liver of offspring of HFD male
Data processing Transcriptome Analysis Console 4.0 Software. Summarization Method: Gene Level-SST-RMA. Gene-Level Fold Change < -1,5 or > 1,5. Gene-Level P-Value < 0,05. Anova Method: ebayes
 
Submission date Nov 16, 2022
Last update date May 01, 2023
Contact name Carlos Castaño
E-mail(s) ccastano1984@gmail.com
Organization name CIBER
Street address Rossello 153
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL24242
Series (1)
GSE218120 Comparison of the effects of paternal obesity upon gene expression in mice

Data table header descriptions
ID_REF
VALUE TAC Sample Signal (Log2)

Data table
ID_REF VALUE
TC0100000014.mm.2 9.94
TC0100000018.mm.2 4.06
TC0100000021.mm.2 8.65
TC0100000022.mm.2 3.71
TC0100000023.mm.2 3.39
TC0100000027.mm.2 14.14
TC0100000038.mm.2 8.29
TC0100000039.mm.2 13.85
TC0100000041.mm.2 3.86
TC0100000044.mm.2 7.62
TC0100000045.mm.2 4.64
TC0100000048.mm.2 10.68
TC0100000055.mm.2 9.08
TC0100000056.mm.2 4.65
TC0100000064.mm.2 5.51
TC0100000071.mm.2 9
TC0100000082.mm.2 3.87
TC0100000092.mm.2 9.66
TC0100000097.mm.2 7.32
TC0100000101.mm.2 5.11

Total number of rows: 22206

Table truncated, full table size 500 Kbytes.




Supplementary file Size Download File type/resource
GSM6734868_LF9_271021_G10.CEL.gz 1002.5 Kb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap