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Sample GSM673794 Query DataSets for GSM673794
Status Public on Jun 17, 2011
Title Male_rep4
Sample type RNA
 
Source name S1 fraction of plasma, male
Organism Homo sapiens
Characteristics tissue: plasma-derived
subfraction: supernatant 1
gender: male
disease state: healthy
Treatment protocol All samples were initially spun at 1700g for 10 minutes to separate the plasma from the Buffy Coat and Red Blood Cells. The plasma was re-centrifuged at 2000g for 10 minutes to obtain the Supernatant 1 (S1) fraction.
Growth protocol Blood was drawn in sodium EDTA tubes and processed within 4 hours of draw.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol-LS, Phenol:Choloroform and purified through Ambion mirVANA columns.
Label biotin
Label protocol A total of 500ng of total RNA was labelled using the Genisphere HSR Labelling Kit (http://www.genisphere.com/array_detection_flashtag_biotin.html).
 
Hybridization protocol The total amount of RNA was hybridized to the arrays for 16 hours at 48C with rotation at 60rpm.
Scan protocol Samples were scanned using the GCS 3000 7G Scanner and processed using miRNA_1-0_2xgain library files.
Description miRNA expression data.
Data processing The data was preprocessed using the Affymetrix miRNA QC tool where Individual feature intensities were flagged by detection calls, background subtracted, transformed through addition of a small constant (value equal to 16), and quantile normalized and median summarized.
 
Submission date Feb 11, 2011
Last update date Jun 17, 2011
Contact name Radha Duttagupta
E-mail(s) Radharani_Duttagupta@affymetrix.com
Phone 408-731-5691
Fax 408-731-5463
Organization name Affymetrix
Department Applied Research
Street address 3450 Central Expressway
City Santa Clara
State/province CA
ZIP/Postal code 95051
Country USA
 
Platform ID GPL8786
Series (2)
GSE27253 Analysis of the impact of cellular miRNA signatures on the profiles of circulating miRNA biomarkers (variability data)
GSE27256 Impact of cellular miRNAs on the biomarker signatures of circulating miRNAs

Data table header descriptions
ID_REF
VALUE Background-subtracted, transformed, quantile-normalized data

Data table
ID_REF VALUE
14q-0_st 3.908547
14qI-1_st 4.513426
14qI-2_st 4.085225
14qI-3_x_st 4.315339
14qI-4_st 4.241512
14qI-4_x_st 4.125274
14qI-5_st 4.125274
14qI-6_st 3.908547
14qI-6_x_st 4.165986
14qI-7_st 4.281127
14qI-8_st 4.085225
14qI-8_x_st 4.165986
14qI-9_x_st 3.908547
14qII-1_st 3.766343
14qII-1_x_st 4.044795
14qII-10_st 4.085225
14qII-11_st 3.999519
14qII-11_x_st 3.908547
14qII-12_st 3.766343
14qII-12_x_st 3.999519

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.




Supplementary file Size Download File type/resource
GSM673794.CEL.gz 154.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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