|
| Status |
Public on Jun 17, 2011 |
| Title |
Male_rep8 |
| Sample type |
RNA |
| |
|
| Source name |
S1 fraction of plasma, male
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: plasma-derived
subfraction: supernatant 1
gender: male
disease state: healthy
|
| Treatment protocol |
All samples were initially spun at 1700g for 10 minutes to separate the plasma from the Buffy Coat and Red Blood Cells. The plasma was re-centrifuged at 2000g for 10 minutes to obtain the Supernatant 1 (S1) fraction.
|
| Growth protocol |
Blood was drawn in sodium EDTA tubes and processed within 4 hours of draw.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol-LS, Phenol:Choloroform and purified through Ambion mirVANA columns.
|
| Label |
biotin
|
| Label protocol |
A total of 500ng of total RNA was labelled using the Genisphere HSR Labelling Kit (http://www.genisphere.com/array_detection_flashtag_biotin.html).
|
| |
|
| Hybridization protocol |
The total amount of RNA was hybridized to the arrays for 16 hours at 48C with rotation at 60rpm.
|
| Scan protocol |
Samples were scanned using the GCS 3000 7G Scanner and processed using miRNA_1-0_2xgain library files.
|
| Description |
miRNA expression data.
|
| Data processing |
The data was preprocessed using the Affymetrix miRNA QC tool where Individual feature intensities were flagged by detection calls, background subtracted, transformed through addition of a small constant (value equal to 16), and quantile normalized and median summarized.
|
| |
|
| Submission date |
Feb 11, 2011 |
| Last update date |
Jun 17, 2011 |
| Contact name |
Radha Duttagupta |
| E-mail(s) |
Radharani_Duttagupta@affymetrix.com
|
| Phone |
408-731-5691
|
| Fax |
408-731-5463
|
| Organization name |
Affymetrix
|
| Department |
Applied Research
|
| Street address |
3450 Central Expressway
|
| City |
Santa Clara |
| State/province |
CA |
| ZIP/Postal code |
95051 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE27253 |
Analysis of the impact of cellular miRNA signatures on the profiles of circulating miRNA biomarkers (variability data) |
| GSE27256 |
Impact of cellular miRNAs on the biomarker signatures of circulating miRNAs |
|
