|
Status |
Public on May 31, 2023 |
Title |
KO1-591 |
Sample type |
SRA |
|
|
Source name |
muscle
|
Organism |
Mus musculus |
Characteristics |
gender: Male cell line: Tarsl2 KO tissue: muscle strain: C57BL/6 age: 6 weeks
|
Treatment protocol |
Mice were feed with standard chow food and water.
|
Growth protocol |
Tarsl2 WT and KO mice were born in SPF animal room and cultured until 6 weeks.
|
Extracted molecule |
total RNA |
Extraction protocol |
Retians were removed, flash frozen on dry ice, and RNA was harvesterd using trizol reagent. Illumina Truseq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Illumina Casava1.7 software used for basecalling Sequence reads were trimmed for adaptor sequence/low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters Reads per kilobase of exon per megabse of library size (RPKM) were calculated using a protocol from chepelev et al., Nucleic Acids Research, Read count extraction and normalization were performed using CLC genomic benchwork Supplementary files format and content: mm8 Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
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|
|
Submission date |
Nov 18, 2022 |
Last update date |
May 31, 2023 |
Contact name |
剑辉 张 |
E-mail(s) |
zhangjianhui2020@sibcb.ac.cn
|
Organization name |
SIBCB
|
Street address |
岳阳路320号细胞楼11楼
|
City |
Shanghai |
ZIP/Postal code |
200031 |
Country |
China |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE218318 |
Tarsl2 is evolved not for tRNA charging in vivo |
|
Relations |
BioSample |
SAMN31785775 |
SRA |
SRX18306626 |