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Sample GSM6741381 Query DataSets for GSM6741381
Status Public on May 31, 2023
Title KOT1-589
Sample type SRA
Source name muscle
Organism Mus musculus
Characteristics gender: Male
cell line: Tarsl2 KO
tissue: muscle
strain: C57BL/6
age: 6 weeks
Treatment protocol Mice were feed with standard chow food and water.
Growth protocol Tarsl2 WT and KO mice were born in SPF animal room and cultured until 6 weeks.
Extracted molecule total RNA
Extraction protocol Retians were removed, flash frozen on dry ice, and RNA was harvesterd using trizol reagent. Illumina Truseq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing Illumina Casava1.7 software used for basecalling
Sequence reads were trimmed for adaptor sequence/low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters
Reads per kilobase of exon per megabse of library size (RPKM) were calculated using a protocol from chepelev et al., Nucleic Acids Research,
Read count extraction and normalization were performed using CLC genomic benchwork
Supplementary files format and content: mm8
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
Submission date Nov 18, 2022
Last update date May 31, 2023
Contact name 剑辉 张
Organization name SIBCB
Street address 岳阳路320号细胞楼11楼
City Shanghai
ZIP/Postal code 200031
Country China
Platform ID GPL13112
Series (1)
GSE218318 Tarsl2 is evolved not for tRNA charging in vivo
BioSample SAMN31785774
SRA SRX18306627

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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