|
Status |
Public on Mar 02, 2023 |
Title |
A032 |
Sample type |
SRA |
|
|
Source name |
Distal Intestine
|
Organism |
Salmo salar |
Characteristics |
tissue: Distal Intestine diet: D3 treatment: HK L-137 Medium inclusion water stage: Fresh water
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from each sample using RNeasy Mini Kit (Qiagen) following the supplier’s instructions. Then, RNA samples were quantified using a NanoDrop™ 8000 spectrophotometer and RNA integrity was measured by Agilent Bioanalyzer 2100. All samples showed an RNA integrity number ≥ 7). RNA libraries for RNA-seq were prepared by Novogene (UK) Company Limited
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
nf-core rnaseq v3.3 Fragment mapping was counted using featureCounts (subread v1.5.1) Differentially expressed genes (DEGs) were estimated using the SARTools R package (v1.7.3). Significant DEGs were determined when the adjusted p-value (padj) was < 0.05. Assembly: SSAL_v3.1 (GenBank assembly accession: GCA_905237065.2). Supplementary files format and content: Count files / Counts for each Sample
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|
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Submission date |
Nov 18, 2022 |
Last update date |
Mar 02, 2023 |
Contact name |
Byron Morales Lange |
E-mail(s) |
byron.maximiliano.morales.lange@nmbu.no
|
Organization name |
Norwegian University of Life Sciences
|
Street address |
Arboretveien 6
|
City |
Ås |
ZIP/Postal code |
1430 |
Country |
Norway |
|
|
Platform ID |
GPL24981 |
Series (1) |
GSE218341 |
From a cells model to a fish trial: Characterization of the immunomodulatory effects of Heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids |
|
Relations |
BioSample |
SAMN31788133 |
SRA |
SRX18311616 |