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Sample GSM6752470 Query DataSets for GSM6752470
Status Public on Jun 07, 2023
Title HepG2 cells, 1uM JTE-607 QuantSeq
Sample type SRA
 
Source name HepG2
Organism Homo sapiens
Characteristics cell line: HepG2
treatment: 1uM JTE-607
Extracted molecule total RNA
Extraction protocol Total cellular RNA was subjected to RNA sequencing by using the QuantSeq FWD kit. Library preparation and sequencing were carried out by Admera Health (South Plainfield, NJ, USA).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing QuantSeq FWD data were processed according to manufacturer’s recommendation. Briefly, raw reads were first trimmed by using the BBtools (Bushnell et al., 2017) and then mapped to the human genome (hg19) using STAR-2.7.7a (Dobin et al., 2013). The number of reads mapped to each gene was counted by using featureCounts (Liao et al., 2014).
Assembly: hg19
Supplementary files format and content: tab-delimited text file including reads count for each pAs/gene
 
Submission date Nov 22, 2022
Last update date Jun 12, 2023
Contact name Bin Tian
E-mail(s) btian@wistar.org
Organization name The Wistar Institute
Street address 3601 Spruce Street
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platform ID GPL11154
Series (2)
GSE218555 Elevated pre-mRNA 3' end processing activity in cancer cells renders vulnerability to inhibition of cleavage and polyadenylation [QuantSeq]
GSE218557 Elevated pre-mRNA 3' end processing activity in cancer cells renders vulnerability to inhibition of cleavage and polyadenylation
Relations
BioSample SAMN31839952
SRA SRX18351991

Supplementary file Size Download File type/resource
GSM6752470_HepG2_JTE1.featurecounts.Rmatrix.txt.gz 272.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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