|
| Status |
Public on Nov 27, 2023 |
| Title |
si-nc_4 [DN4] |
| Sample type |
SRA |
| |
|
| Source name |
BMDC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: BMDC
genotype: wild type
strain: C57/BL6
treatment: si-nc
|
| Growth protocol |
Bone marrow cells from C57/BL6 mice were cultured with GM-CSF and IL-4 for 6 days and stimulated with LPS for 24 hours to generate bone-marrow derived DC (BMDC). CD11c+ BMDC were isolated by anti-mouse CD11c magbeads to generate maDC. maDC were then cocultured with spleen stroma cells for 9 days to generate diffDC. And then diffDCs were transfected with si-Suclg2 or si-nc.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
diffDCs transfected with si-Suclg2 or si-nc were collected for RNA extraction, total RNA was extracted with TRIzol reagent.
cDNA library construction and sequencing were performed by Beijing Genomics Institute using Illumina HiSeq platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
cDNA library construction and sequencing were performed by Beijing Genomics Institute using Illumina HiSeq platform.
High-quality reads were aligned to the mouse reference genome GRCm38.p4 (mm10).
The expression levels for each of the genes were normalized to fragments per kilobase of exon model per million mapped reads (FPKM).
Assembly: GRCm38.p4 (mm10)
Supplementary files format and content: diffDC transfected with si-nc or si-Suclg2 mRNA.GeneDiffExp.xlsx
|
| |
|
| Submission date |
Dec 04, 2022 |
| Last update date |
Nov 27, 2023 |
| Contact name |
xiaomin zhang |
| E-mail(s) |
xiaoxiao0666@126.com
|
| Phone |
18217743328
|
| Organization name |
National Key Laboratory of Medical Immunology & Institute of Immunology, Naval Military Medical University
|
| Street address |
800 Xiang Yin Road
|
| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE220055 |
Suclg2 maintains the tolerogenic function of regulatory dendritic cells by suppressing Lactb succinylation [si-nc/si-Suclg2] |
|
| Relations |
| BioSample |
SAMN32033760 |
| SRA |
SRX18487394 |
