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Sample GSM6784431 Query DataSets for GSM6784431
Status Public on Nov 27, 2023
Title si-Suclg2_2 [DS2]
Sample type SRA
 
Source name BMDC
Organism Mus musculus
Characteristics cell type: BMDC
genotype: wild type
strain: C57/BL6
treatment: si-Suclg2
Growth protocol Bone marrow cells from C57/BL6 mice were cultured with GM-CSF and IL-4 for 6 days and stimulated with LPS for 24 hours to generate bone-marrow derived DC (BMDC). CD11c+ BMDC were isolated by anti-mouse CD11c magbeads to generate maDC. maDC were then cocultured with spleen stroma cells for 9 days to generate diffDC. And then diffDCs were transfected with si-Suclg2 or si-nc.
Extracted molecule total RNA
Extraction protocol diffDCs transfected with si-Suclg2 or si-nc were collected for RNA extraction, total RNA was extracted with TRIzol reagent.
cDNA library construction and sequencing were performed by Beijing Genomics Institute using Illumina HiSeq platform.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing cDNA library construction and sequencing were performed by Beijing Genomics Institute using Illumina HiSeq platform.
High-quality reads were aligned to the mouse reference genome GRCm38.p4 (mm10).
The expression levels for each of the genes were normalized to fragments per kilobase of exon model per million mapped reads (FPKM).
Assembly: GRCm38.p4 (mm10)
Supplementary files format and content: diffDC transfected with si-nc or si-Suclg2 mRNA.GeneDiffExp.xlsx
 
Submission date Dec 04, 2022
Last update date Nov 27, 2023
Contact name xiaomin zhang
E-mail(s) xiaoxiao0666@126.com
Phone 18217743328
Organization name National Key Laboratory of Medical Immunology & Institute of Immunology, Naval Military Medical University
Street address 800 Xiang Yin Road
City shanghai
ZIP/Postal code 200433
Country China
 
Platform ID GPL13112
Series (1)
GSE220055 Suclg2 maintains the tolerogenic function of regulatory dendritic cells by suppressing Lactb succinylation [si-nc/si-Suclg2]
Relations
BioSample SAMN32033758
SRA SRX18487396

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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