NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM679710 Query DataSets for GSM679710
Status Public on Oct 05, 2011
Title Bone marrow transduced with CALM-AF10, biological replicate-1
Sample type RNA
 
Source name Adult mouse bone marrow
Organism Mus musculus
Characteristics strain: C57/BL6/C3H
cell type: bone marrow
transducer: CALM-AF10
Treatment protocol After 48 hours of pre-stimulation, the BM cells were transduced with different viruses, containing the relevent constructs, by overlaying them on virus-producing irradiated (40 Gy) GFP+E86 producers in the presence of cytokines and protamine sulfate (5ug/ml). The transduction was stopped by removing the BM cells from the GFP+E86 cells and plating them in BM medium with cytokines for another 48 hours to allow for GFP expression. Retrovirally transduced cells were FACS sorted based on expression of GFP.
Growth protocol Adult C57/BL6/C3H mice were injected with 30µg/µl working solution of 5-Fluorouracil (100µl per 20gr of weight) to enrich for stem and progenitor cells. Four days post injection, total bone marrow
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FACS sorted GFP+ cells using the Qiagen miRNeasy Mini Kit For purification of microRNA and total RNA from tissues and cells per manufacturer's instructions
Label biotin
Label protocol Whole Transcript (WT) Sense Target Labeling Assay
 
Hybridization protocol according to Affymetrix Whole Transcript (WT) Sense Target Labeling Assay
Scan protocol according to Affymetrix protocols using a GeneChip 3000 Scanner
Description Gene expression data from bone marrow cells 96 hours post-transdution with CALM-AF10
Data processing Affymetrix® Expression Console™ Software for normalization, BRB Array Tools for statistical analysis
 
Submission date Feb 24, 2011
Last update date Oct 05, 2011
Contact name Karlheinz Holzmann
E-mail(s) karlheinz.holzmann@uni-ulm.de
Organization name Ulm University
Department Medical Faculty
Lab Genomics Core Facility
Street address Helmholtzstreet 8/1
City Ulm
ZIP/Postal code D-89081
Country Germany
 
Platform ID GPL6246
Series (2)
GSE27513 Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA)
GSE27514 Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant

Data table header descriptions
ID_REF
VALUE RMA normalized, log2

Data table
ID_REF VALUE
10344614 7.079818
10344616 3.312201
10344620 4.469812
10344622 7.823535
10344624 10.61036
10344633 10.15423
10344637 8.901014
10344653 4.423347
10344658 8.55191
10344674 4.475738
10344679 5.659426
10344707 8.513093
10344713 11.05712
10344715 4.54283
10344717 4.031384
10344719 6.108578
10344721 3.233489
10344723 8.315589
10344725 6.330485
10344741 10.19143

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM679710.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap