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Status |
Public on Oct 05, 2011 |
Title |
Bone marrow transduced with CALM-AF10, biological replicate-1 |
Sample type |
RNA |
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|
Source name |
Adult mouse bone marrow
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Organism |
Mus musculus |
Characteristics |
strain: C57/BL6/C3H cell type: bone marrow transducer: CALM-AF10
|
Treatment protocol |
After 48 hours of pre-stimulation, the BM cells were transduced with different viruses, containing the relevent constructs, by overlaying them on virus-producing irradiated (40 Gy) GFP+E86 producers in the presence of cytokines and protamine sulfate (5ug/ml). The transduction was stopped by removing the BM cells from the GFP+E86 cells and plating them in BM medium with cytokines for another 48 hours to allow for GFP expression. Retrovirally transduced cells were FACS sorted based on expression of GFP.
|
Growth protocol |
Adult C57/BL6/C3H mice were injected with 30µg/µl working solution of 5-Fluorouracil (100µl per 20gr of weight) to enrich for stem and progenitor cells. Four days post injection, total bone marrow
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from FACS sorted GFP+ cells using the Qiagen miRNeasy Mini Kit For purification of microRNA and total RNA from tissues and cells per manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Whole Transcript (WT) Sense Target Labeling Assay
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|
|
Hybridization protocol |
according to Affymetrix Whole Transcript (WT) Sense Target Labeling Assay
|
Scan protocol |
according to Affymetrix protocols using a GeneChip 3000 Scanner
|
Description |
Gene expression data from bone marrow cells 96 hours post-transdution with CALM-AF10
|
Data processing |
Affymetrix® Expression Console™ Software for normalization, BRB Array Tools for statistical analysis
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|
|
Submission date |
Feb 24, 2011 |
Last update date |
Oct 05, 2011 |
Contact name |
Karlheinz Holzmann |
E-mail(s) |
karlheinz.holzmann@uni-ulm.de
|
Organization name |
Ulm University
|
Department |
Medical Faculty
|
Lab |
Genomics Core Facility
|
Street address |
Helmholtzstreet 8/1
|
City |
Ulm |
ZIP/Postal code |
D-89081 |
Country |
Germany |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE27513 |
Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA) |
GSE27514 |
Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant |
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