|
| Status |
Public on Jun 08, 2023 |
| Title |
BT549--pcIp2 |
| Sample type |
SRA |
| |
|
| Source name |
BT549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BT549
cell type: breast cancer cells
genotype: vector control
|
| Growth protocol |
BT549-SNHGI5 and BT549-pcIP2 Cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100U/ml penicillin, and 100 µg/ml streptomycin at 37 °C in a humid environment with 5 % CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using MJzol animal RNA Extraction Kit(MagBeads) (Cat#T102096)following the manufacturer’s instructions.
RNA libraries for RNA-seq were prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
mRNA profiling by RNA sequencing
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and low-quality sequence using Seqtk.
Clean reads were compared with hg38.The analysis was performed by the Shanghai Biotechnology Corporation.
Assembly: hg38
Supplementary files format and content: Tab-delimited files include FPKM values
|
| |
|
| Submission date |
Dec 08, 2022 |
| Last update date |
Jun 08, 2023 |
| Contact name |
Zhou Yanchun |
| E-mail(s) |
zyc2013st@foxmail.com
|
| Organization name |
Shantou University Medical College
|
| Department |
Pathophysiology
|
| Street address |
Xinling Road
|
| City |
Shantou |
| State/province |
Guangdong |
| ZIP/Postal code |
515041 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE220492 |
Transcriptome analysis of BT549 cells overexpressing SNHG15 |
|
| Relations |
| BioSample |
SAMN32109472 |
| SRA |
SRX18537383 |
