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Sample GSM6822465 Query DataSets for GSM6822465
Status Public on Jun 07, 2023
Title 16HBE14o- CFTRhigh #4 viewpoint_+48.9kb rep2 [4C-seq]
Sample type SRA
 
Source name 16HBE14o-
Organism Homo sapiens
Characteristics cell line: 16HBE14o-
cell type: human bronchial epithelial cells
genotype: CFTRhigh
Growth protocol Cells were grown in DMEM + 10% FBS using standard methods
Extracted molecule genomic DNA
Extraction protocol Nuclei from 1e7 cells grown to confluency were made as per Krijger, P et al, 2020, Methods (PMID: 31351925).
All libraries were constructed with Illumina sequencing adapters as per PMID: 31351925.
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model NextSeq 550
 
Description 4C-seq library with the CFTR +48.9 kb region as the viewpoint
Data processing library strategy: 4C-seq
Libraries were sequenced on the Nextseq550 machine and demultiplexed using the primer sequences.
4C-seq was quantified and mapped using the pipe4C v1.1 (https://github.com/deLaatLab/pipe4C) processing pipeline using default parameters, aligned to the hg19 genome.
Assembly: hg19
Supplementary files format and content: Aligned 4C-seq of each sample of at a single viewpoint in bigWig format
 
Submission date Dec 13, 2022
Last update date Jun 07, 2023
Contact name Ann Harris
E-mail(s) axh575@case.edu
Organization name Case Western Reserve University
Department Genetics and Genome Sciences
Street address 2109 Adelbert Rd
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
 
Platform ID GPL21697
Series (2)
GSE220852 Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]
GSE220855 Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts.
Relations
BioSample SAMN32192591
SRA SRX18671770

Supplementary file Size Download File type/resource
GSM6822465_16HBE_SC_4high_489_AH1417.bw 5.1 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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