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Sample GSM6822479 Query DataSets for GSM6822479
Status Public on Jun 07, 2023
Title 16HBE14o- CFTRlow #12 viewpoint_-20.9kb rep1 [4C-seq]
Sample type SRA
Source name 16HBE14o-
Organism Homo sapiens
Characteristics cell line: 16HBE14o-
cell type: human bronchial epithelial cells
genotype: CFTRlow
Growth protocol Cells were grown in DMEM + 10% FBS using standard methods
Extracted molecule genomic DNA
Extraction protocol Nuclei from 1e7 cells grown to confluency were made as per Krijger, P et al, 2020, Methods (PMID: 31351925).
All libraries were constructed with Illumina sequencing adapters as per PMID: 31351925.
Library strategy OTHER
Library source genomic
Library selection other
Instrument model NextSeq 550
Description 4C-seq library with the CFTR -20.9 kb region as the viewpoint
Data processing library strategy: 4C-seq
Libraries were sequenced on the Nextseq550 machine and demultiplexed using the primer sequences.
4C-seq was quantified and mapped using the pipe4C v1.1 ( processing pipeline using default parameters, aligned to the hg19 genome.
Assembly: hg19
Supplementary files format and content: Aligned 4C-seq of each sample of at a single viewpoint in bigWig format
Submission date Dec 13, 2022
Last update date Jun 07, 2023
Contact name Ann Harris
Organization name Case Western Reserve University
Department Genetics and Genome Sciences
Street address 2109 Adelbert Rd
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
Platform ID GPL21697
Series (2)
GSE220852 Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts [4C-seq]
GSE220855 Cellular heterogeneity in the 16HBE14o- airway epithelial line impacts functional readouts.
BioSample SAMN32192578
SRA SRX18671783

Supplementary file Size Download File type/resource 5.0 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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