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Sample GSM6879231 Query DataSets for GSM6879231
Status Public on Jun 07, 2023
Title K4
Sample type SRA
 
Source name AngII/PE treated male
Organism Mus musculus
Characteristics cell type: Whole LV myocardium
genotype: WT C57Bl6J/129X1 hybrid
treatment: AngII/PE infusion for 3 days
Sex: male
time: 3 days
Extracted molecule total RNA
Extraction protocol RNA was harvested using Ambion PureLink RNA minikit, lot 2379554, following manufacturer's protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing RNA-Seq reads were trimmed by cutadapt first, then trimmed read were aligned against mouse genome (mm10) using STAR v2.4.2a. Gene counts were derived from the number of uniquely aligned unambiguous reads by Subread: featureCount with mouse GENCODE gene annotation (M15).
All gene-level transcript counts were then imported into DESeq2, and normalization method was used to adjust for differences in library size
Assembly: mm10
Supplementary files format and content: xlsx
 
Submission date Dec 21, 2022
Last update date Jun 07, 2023
Contact name Tiandao Li
Organization name Washington University
Street address 4444 Forest Park Ave
City St Louis
State/province MO
ZIP/Postal code 63108
Country USA
 
Platform ID GPL17021
Series (1)
GSE221502 Characterization of a robust mouse model for heart failure with preserved ejection fraction
Relations
BioSample SAMN32347893
SRA SRX18804614

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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