|
| Status |
Public on Dec 14, 2011 |
| Title |
hHFF SM3 |
| Sample type |
RNA |
| |
|
| Source name |
Post natal fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HFFSM3
cell type: fibroblast
|
| Growth protocol |
Human post-natal fibroblasts from a 4 year old donor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy mini kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer
|
| Label |
Biotin
|
| Label protocol |
Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) protocol ‘small sample protocol II’, starting from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 11, 2011 |
| Last update date |
Dec 14, 2011 |
| Contact name |
Jean-Marc Lemaitre |
| E-mail(s) |
Jean-Marc.Lemaitre@igf.cnrs.fr
|
| Organization name |
INSERM
|
| Street address |
141 rue de la cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34094 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27924 |
Microarray data for rejuvenation experiments through reprogramming |
|
