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Sample GSM6909004 Query DataSets for GSM6909004
Status Public on May 04, 2023
Title mESC_WT rep2
Sample type SRA
 
Source name embryonic stem cells (ESCs)
Organism Mus musculus
Characteristics strain: 129S5/SvEvBrd
cell type: ESCs
genotype: Control
Treatment protocol ESCs were treated with 50 μM Mdivi-1 for 24h.
Growth protocol The mouse ESC line AB2.2 were seeded on mitotically inactivated MEF feeder cells in N2B27 medium with 2i and LIF in tissue culture dishes.
Extracted molecule total RNA
Extraction protocol For mouse ESCs, total RNA was extracted by TRIzol reagent (Invitrogen, USA) from 5×105 cells for each group.
RNA libraries were prepared for sequencing using standard RNA-seq library preparation (Truseq RNA Sample Preparation Kit, Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Data processing Raw reads were processed with Cutadapt v1.16. Trimmed reads were mapped to mouse genome using STAR with default settings.
Assembly: mm10
Supplementary files format and content: DESeq result with normalized and raw gene counts for every gene and every sample
 
Submission date Dec 29, 2022
Last update date May 04, 2023
Contact name Li-quan Zhou
E-mail(s) zhouliquan@hust.edu.cn
Organization name huazhong university of science and technology
Street address 13 Hangkong road
City Wuhan
State/province Hubei
ZIP/Postal code 430030
Country China
 
Platform ID GPL21493
Series (2)
GSE221885 Mdivi1 treatment impacts transcriptome of mouse embryonic stem cells
GSE221989 Mdivi1 treatment impacts mouse embryonic stem cells and early embryos.
Relations
BioSample SAMN32489901
SRA SRX18881978

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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