|
Status |
Public on May 04, 2023 |
Title |
mESC_WT rep2 |
Sample type |
SRA |
|
|
Source name |
embryonic stem cells (ESCs)
|
Organism |
Mus musculus |
Characteristics |
strain: 129S5/SvEvBrd cell type: ESCs genotype: Control
|
Treatment protocol |
ESCs were treated with 50 μM Mdivi-1 for 24h.
|
Growth protocol |
The mouse ESC line AB2.2 were seeded on mitotically inactivated MEF feeder cells in N2B27 medium with 2i and LIF in tissue culture dishes.
|
Extracted molecule |
total RNA |
Extraction protocol |
For mouse ESCs, total RNA was extracted by TRIzol reagent (Invitrogen, USA) from 5×105 cells for each group. RNA libraries were prepared for sequencing using standard RNA-seq library preparation (Truseq RNA Sample Preparation Kit, Illumina).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Data processing |
Raw reads were processed with Cutadapt v1.16. Trimmed reads were mapped to mouse genome using STAR with default settings. Assembly: mm10 Supplementary files format and content: DESeq result with normalized and raw gene counts for every gene and every sample
|
|
|
Submission date |
Dec 29, 2022 |
Last update date |
May 04, 2023 |
Contact name |
Li-quan Zhou |
E-mail(s) |
zhouliquan@hust.edu.cn
|
Organization name |
huazhong university of science and technology
|
Street address |
13 Hangkong road
|
City |
Wuhan |
State/province |
Hubei |
ZIP/Postal code |
430030 |
Country |
China |
|
|
Platform ID |
GPL21493 |
Series (2) |
GSE221885 |
Mdivi1 treatment impacts transcriptome of mouse embryonic stem cells |
GSE221989 |
Mdivi1 treatment impacts mouse embryonic stem cells and early embryos. |
|
Relations |
BioSample |
SAMN32489901 |
SRA |
SRX18881978 |